离心超滤装置用于浓缩和澄清过滤生物样品，蛋白纯化，颗粒物去除，血浆或血清过滤，以及从细胞裂解液或细胞培养上清去除细胞或细胞碎片。
Sartorius Stedim Biotech提供一系列离心设备，快速安全处理100 μl到100ml样品。
可选抛弃型和可重复使用超滤装置。
Overview：
· High flux PES membrane
· High retentate recovery > 90 %
· Vertical membrane design for ultra fast concentration
· Universal rotor compatibility
· Safe sample concentration due to dead-stop volume
· Convenient concentrate recovery by pipetting
· Volume graduation for easy reading/monitoring
Description
Vivaspin centrifugal concentrators are disposable ultrafiltration devices for the concentration of biological samples. Vivaspin 500 centrifugal filter units offer a simple, one step procedure for sample concentration with starting volumes up to 500 μl. They can effectively be used in fixed angle rotors accepting 2.2 ml centrifuge tubes. The patented vertical membrane design and thin channel filtration chamber (US 5,647,990), minimises membrane fouling and provides high speed concentrations. Vivaspin 500 units are available with PES membrane in a wide range of molecular weight cut-offs. Concentrate recoveries of > 90% can be reached in less than 30 min. The dead stop pocket prevents concentration to dryness.
Performance Characteristics
| Time to concentrate up to 30x [min.] at 20°C | Concentrate recovery % |
Start volume | 500 μl | 500 μl |
Aprotinin 0.25 mg/ml (6,500 MW) |
|
|
3,000 MWCO PES | 30 | 96 % |
BSA 1.0 mg/ml (66,000 MW) |
|
|
5,000 MWCO PES | 15 | 96 % |
10,000 MWCO PES | 5 | 96 % |
30,000 MWCO PES | 5 | 95 % |
IgG 0.25 mg/ml (160,000 MW) |
|
|
30,000 MWCO PES | 10 | 96 % |
50,000 MWCO PES | 10 | 96 % |
100,000 MWCO PES | 10 | 96 % |
技术规格：
产品特性
Market | BioPharma;Research |
应用 | Concentration;Desalting | buffer exchange |
样品类型 | Nanoparticles;Protein;Virus |
Ultrafiltration device type | Centrifugal Ultrafiltration Devices |
MWCO | 30.000 Dalton |
Membrane | Polyethersulfone (PES) |
Type | Centrifugal unit |
Volume Range | 0,1 - 0,5 ml |
Certification | None |
技术属性
Fixed Angle Rotor | 600 μl |
Width | 338 mm |
Active Membrane Area | 0,5 cm2 |
Body | Polycarbonate |
Filtrate vessel | Polypropylene (PP) |
Concentrator Cap | Polycarbonate |
Membrane material | Polyethersulfone (PES) |
Hold-up volume | < 5 μl |
Overall length | 50 mm |
Dead stop volume | 5 μl |